monoclonal antibody rc2  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: The valine-arginine dipeptide repeat protein encoded by mammalian telomeric RNA appears highly expressed in mitosis and may repress global translation

doi: 10.1101/2024.07.24.604971

Figure Lengend Snippet: (A) E16 mouse embryonic cortical section was labeled with anti-VR (teal) and cortical progenitor-specific RC2 (Yellow) antibodies. VR labels the ventricular zone (VZ) where progenitors proliferate as well as the cortical plate (CP) where new neurons settle. (B-C) VR expression is also evident in the early (E14) and late-stage (P0) cortical ventricular zone. Anti-VR and RC2 antibodies were used at 1:200 dilution. IZ, intermediate zone. Scale bar: 50µm (A), 25µm (B-C).

Article Snippet: The following primary antibodies were used: anti-VR ( ) and RC2 (1:200 dilution; mouse IgM, Developmental Studies Hybridoma Bank, University of Iowa).

Techniques: Labeling, Expressing

Journal: PLoS ONE

Article Title: Dab1 ( Disable Homolog-1 ) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages

doi: 10.1371/journal.pone.0026673

Figure Lengend Snippet: List of antibodies and cell types labelled.

Article Snippet: RC2 antibody was detected by incubating 2 h at RT with biotin conjugated goat anti-mouse IgM (Jackson Immunoresearch) and then incubated 1 h at RT with Alexa 568 conjugated streptavidin (Molecular Probes-Invitrogen).

Techniques:

Journal: PLoS ONE

Article Title: Dab1 ( Disable Homolog-1 ) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages

doi: 10.1371/journal.pone.0026673

Figure Lengend Snippet: Extent of Dab1 coexpression with neuronal and glial markers at different postnatal ages in wt and reeler animals.

Article Snippet: RC2 antibody was detected by incubating 2 h at RT with biotin conjugated goat anti-mouse IgM (Jackson Immunoresearch) and then incubated 1 h at RT with Alexa 568 conjugated streptavidin (Molecular Probes-Invitrogen).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Dab1 ( Disable Homolog-1 ) Reelin Adaptor Protein Is Overexpressed in the Olfactory Bulb at Early Postnatal Stages

doi: 10.1371/journal.pone.0026673

Figure Lengend Snippet: Dab1 (green), Map2a,b (red) (a–b, f–g, k–l, p–q) and the radial glial cell marker RC2 (red) (c–e, h–j, m–o, r–t). OB sagittal sections at P0 (a–e), P3 (f–j), P7 (k–o) and P15 (p–t). At P0 neuronal Dab1 positive processes (Map2a,b) are evident in the GL, EPL and MCL (a, b) while radial glial Dab1 positive processes (RC2) occupied the OB surface (c–e). From P3 onwards, neuronal Dab1 positive neuronal fibers become evident in the IPL and GcL (f–g) while the radial cell processes decreased in quantity. Some Rc2 positive/Dab1 negative cells surround the glomeruli (h–j). Neuronal fibers pattern is similar at P7 (k–i) and P15 (p–q), whereas Rc2 fibers are absent from P7 (m–o). From P7 to P15 all radial glial cells are Dab1 negative (m–o; r–t). Nuclei counterstained with Hoechst (blue). ONL, olfactory nerve layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cells layer; IPL, internal plexiform layer; GcL, granular cells layer; SEZ, subependymal zone. Scale bar: 100 µm.

Article Snippet: RC2 antibody was detected by incubating 2 h at RT with biotin conjugated goat anti-mouse IgM (Jackson Immunoresearch) and then incubated 1 h at RT with Alexa 568 conjugated streptavidin (Molecular Probes-Invitrogen).

Techniques: Marker